Scientists have used fluorescence microscopy to examine the dynamics of membrane proteins before, says team leader Simon Scheuring of the French National Institute of Health & Medical Research. But those experiments required that the protein be fluorescently labeled, he says. They also typically followed the motion of only one protein at a time. Scheuring’s team sees all the proteins in the membrane with AFM, he notes, and therefore learns how the diffusion of a protein is affected by its molecular environment. To make the AFM measurements, Scheuring and colleagues deposited the Escherichia coli membrane protein OmpF, along with some E. coli lipids, onto a mica support.
by Lauren K. Wolf |
July 16, 2012