Product Info Now: February 2004
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The role of palladium as a catalyst in organic chemistry has been well documented,
including its use in such common reactions as Suzuki-style cross-coupling reactions,
Heck reactions, and Buchwald amidations, as well as hydrogenations. Although palladium
is recognized as an excellent catalyst, removing it from the target product is
sometimes a problem, and for use in drug discovery, the active compound must contain
palladium levels below a few parts per million. Traditional methods of removing
palladium involve chromatography, but this can be labor- and solvent-intensive.
One modern technique for purifying reactions in drug discovery is the use
of polymer-bound scavenger reagents. Two new palladium scavenger resins have been
developed with which greater than 99% removal of palladium has been demonstrated
by simply stirring either of them with a solution of the compound of interest
at room temperature for 424 h.
A typical scavenging procedure is to add 2.2 molar equivalents of either PS-2-mercaptonicotinamide
(1) or PS-dithiothreitol (2) to the target molecule dissolved in a tetrahydrofuran
(THF) solution. The suspension is either agitated or stirred for 424 h and
then filtered. The polymer reagent is washed twice with THF and filtered. The
combined THF filtrates are then concentrated in vacuo to yield the final product.
Test reactions used 22 mg of palladium acetate [Pd(OAc)2] and 220 mg
of scavenging resin. After 424 h of agitation, less than 1 mg of mass was
recovered. When tetrakis(triphenylphosphine)palladium(0) [Pd(Ph3P)4]
was used as the palladium source, only triphenylphosphine [Ph3P] was
recovered as determined by 1H-NMR spectroscopy and elemental analysis.
Sigma-Aldrich - www.sigmaaldrich.com
The Veloce micro parallel liquid chromatography (mPLC) system provides increased
sample analysis capacity to scientists who need information to make timely research
decisions. It uses established HPLC techniques, integrating them with conventional
laboratory workflow to increase time and cost efficiency. The Veloce system is
designed for fast, high-quality sample analysis in analytical chemistry, specifically
in the drug discovery and development process.
The features of the Veloce system include increased sample analysis capacity
and the ability to generate chromatograms comparable to those produced by conventional
HPLC instrumentation for a broad class of analytical chemistry applications. The
Veloce system provides up to 24 simultaneous separations. This parallel analysis
provides the ability to analyze a large number of compounds simultaneously, generate
a standard curve, and increase the number of replicates or conditions used in
At the heart of the Veloce system is the 24-column Brio cartridge, which enables
multiple samples to be analyzed in parallel for increased throughput. The cartridges
can incorporate columns of varying lengths and a range of stationary phases. Researchers
can select cartridges to fulfill throughput and to optimize performance for specific
experimental protocols. Because the Veloce system is similar to traditional HPLC
systems, scientists can continue to use their existing processes and protocols
when adopting this product.
Nanostream - www.nanostream.com
Membrane filters. Supor EKV 0.2 micron
sterilizing-grade filters are designed for economical filtration of buffers, biologicals,
tissue culture media, ophthalmic products, and many other biopharmaceutical fluids.
These unique poly(ether sulfone) (PES) membrane filters contain a built-in prefilter
layer that has been developed with a proprietary, asymmetrical, V-shaped, funnel-like
pore structure that increases throughput to extend filter life and allows for
economical design of filtration systems. Supor EKV filters will be on display
at INTERPHEX 2004, March 1618, in New York, Booth 1321.
Pall Corp. - www.pall.com
Autosampler. The Sample Sentinel refrigerated
unit offers robotic liquid-chromatographic autosampling by moving the sample vial
to sampling, diluting, or mixing stations without requiring complex mechanical
drivers. Capable of transferring 105 vials (300 µL or 1.8 mL) in three removable
trays when cooled or 120 vials when uncooled, the unit is designed for use with
microbore, standard-bore, or preparative applications and is available for precise
fixed-loop and variable-loop injection. With the sample preparation option, up
to three reagent additions are programmable in microliter volumes with virtually
unlimited programmable steps.
BASi - www.bioanalytical.com
Online platform. The Celera Discovery System
online platform is a subscription-based, integrated research platform that provides
online access to the most comprehensive and current set of curated biological
data and analysis tools available for comparing the human and mouse genomes. The
platform includes an interactive, customizable Map Viewer that provides graphical
displays for comparing human and mouse genes within their genomic contexts.
Applied Biosystems - www.appliedbiosystems.com
Reagents. The Qtracker 655 Kit is designed
for loading Qdot nanocrystals into live cells. The kit uses custom targeting peptides
to deliver highly luminescent Qdot quantum dots to the cytoplasm. Once inside
the cells, Qtracker labels provide strong, stable fluorescence that does not bleach
over time, even after extensive continuous illumination and observation. Qtracker
quantum dots add no additional autofluorescence, are not transferred to adjacent
cells in the population, and display excellent retention properties; the nanocrystals
used in the kit remain inert and nontoxic to many cell types and can be followed
in some cell lines for up to a week.
Quantum Dot Corp. - www.qdots.com
Reagents. Brilliant SYBR Green-based QRT-PCR
reagents provide a universal solution to real-time PCR detection and gene quantification.
The SYBR Green dye binds specifically to double-stranded PCR products without
the need for sequence-specific probes. The QRT-PCR reagents are optimized on Stratagene’s
Mx4000 multiplex quantitative PCR system and Mx3000 real-time PCR system, and
are validated on most other QPCR instruments.
Stratagene - www.stratagene.com
Software. FlashMaster flash chromatography
software supports the purification requirements of several chemists. Common to
both the FlashMaster II and Solo instrument platforms, FlashMaster software performs
real-time chromatography data analysis and instructs the instruments to collect
targeted fractions in minimal solvent volumes. A flash chromatography setup wizard
automatically sets the correct flow rate based on column size, and it guides users
through purification, detection, and fraction collection programming.
Argonaut Technologies - www.argotech.com
Pipettor. The ergonomically designed Scienceware
Pipette Pump III helps to reduce the risk of injury from repetitive and awkward
pipetting motions. The compact and lightweight design adds only 152 mm to the
pipette length and is ideal for fume hoods and controlled-environment glove boxes.
The optimally located thumbwheel aspirates and dispenses down to a volume of one
drop at a time while providing a more relaxed grip.
Bel-Art Products - www.belart.com
Software. The ActiveSite software add-on
to the BioCAChe, BioMed-CAChe, and WorkSystem Pro products provides powerful tools
to speed the drug discovery process by allowing researchers to predict and select
better-quality leads before making them in the laboratory. Scientists can import
and display 3D proteins directly from the Protein Data Bank and then view, analyze,
and modify them by using interactive sequence and 3D structure editor windows.
Users also can optimize proteins, dock ligands, and model reactions of molecules
with tens of thousands of atoms.
CAChe Group - www.cachesoftware.com
Catalog. The 20042005 Intermediates
and Building Blocks Catalog offers 2289 diverse compounds that are available
in multigram quantities and are suitable for combinatorial and other synthetic
requirements. Included in the catalog is a CD-ROM database containing more than
40,000 unique and varied screening compounds and the recently acquired Merlin
database. A copy may be ordered by e-mail at catalogueMDD@keyorganics.ltd.uk.
Key Organics Ltd. - www.keyorganics.ltd.uk
Filter plates. The one-piece design of
MultiScreenHTS filter plates, specifically developed for automated workstations,
features rigid sidewalls for robotic gripper arms, providing ample surface to
accommodate bar-code labels. In addition, a removable underdrain allows easy membrane
access. The high-throughput plates are optimized for general sample preparation,
ELISpot, whole-organism visualization, and radioisotope detection applications.
MultiScreenHTS filter plates are available in a range of plastics and membranes.
Millipore - www.millipore.com
Dispenser. The WellMate is a high-speed,
small-footprint, 8-channel fluid dispenser for 96- and 384-well microplates. It
features a height-adjustable dispense head to accommodate dispensing to shallow-
and deep-well plates and blocks; low-cost, disposable, presterilized tubing cartridges
that enable dispense volumes of 2.02000 µL; programmability in 1.0-µL
increments; memory for storing as many as 18 dispense protocols; and a digital
keypad. RS232 programming promotes robotic integration, and a removable plate
stage makes cleaning fast and easy.
Matrix Technologies - www.matrixtechcorp.com
DNA purification. NucleoSpin Plasmid QuickPure
Kits are designed for batch preparation of up to 15 µg of plasmid DNA from
13 mL of bacterial overnight cultures. These kits allow you to quickly prepare
high-quality plasmid DNA. Simply apply the supernatant of your bacterial lysate
to the column, use a single wash step to remove remaining contaminants, and elute.
The optimized buffer system ensures that contaminants such as salts, metabolites,
nucleases, and soluble cellular components are removed.
BD Biosciences Clontech - www.bdbiosciences.com
Illumination system. The MT20 is an integral
part of a real-time imaging station for live-cell experiments. The system optionally
supports two types of short-arc lamps: 150-W Xe or Hg/Xe mixed-gas burners. Bulbs
can be exchanged within a minute and realigned easily using an integrated photodiode
to monitor light output. The MT20 can be equipped with up to eight excitation
filters of standard size and also features an attenuation device and a very fast
Olympus BioSystems - www.olympusbiosystems.com
PCR system. The FailSafe Real-Time PCR
System incorporates SYBR Green I dye for detecting double-stranded DNA generated
during PCR. During each phase of DNA synthesis, the SYBR Green I dye binds to
the amplified PCR products, and the amplicon quantity is monitored in real time
by measuring its fluorescence. The incorporation of SYBR Green I dye into a real-time
PCR reaction provides great flexibility because no target-specific probes are
Epicentre - www.epicentre.com
Sealing kit. The 500-µL WebSeal Kit
consists of a 96-well, middepth polypropylene Microtiter plate that is preinserted
with 500-µL glass vials and includes the WebSeal silicon/PTFE sealing mat.
The sealing mat provides for easy sealing of all 96 wells to eliminate cross-contamination
of the samples. A cutting tool is provided that allows individual tubes to be
removed from the rack without breaking the seal. The sealing mats are available
solid or pre-slit for easy penetration, and they are dry-heat-autoclavable.
Sun-Sri - www.sun-sri.com
Catalog. This 16-page color catalog includes
digital, programmable, and orbital mixing chilling/heating dry baths. A comprehensive
range of accessory sample blocks for centrifuge tubes, PCR tubes, assay plates,
and test tubes is also available. In addition, the catalog offers digital and
programmable chilling/heating benchtop incubators, digital chilling/heating HPLC
column ovens, digital chilling/heating minicirculators, and variable-speed rotating
mixers. To order, e-mail email@example.com.
Torrey Pines Scientific - www.torreypinesscientific.com
Syringe pump. The Baby Bee model offers
a unique alternative to traditional syringe pump design. Because the syringe drive
is unbundled from the control mechanism, the pump is smaller and much more versatile,
since a variety of controllers can be used for the same syringe drive. This approach
eliminates the cost (in both dollars and laboratory space) of redundant power
supplies, displays, and controls common to pump designs in which the syringe drive
is built into the control cabinet.
BASi - www.bioanalytical.com
Pipettor. The Cellmate II, a portable serological
pipettor, is powered by a lithium ion battery and comes with a table stand, wall
mount, universal power supply, and plug adapters for international use. Large
aspirate and dispense triggers offer a comfortable finger platform and allow precise
control of liquid manipulations. A large, backlit LED monitors battery charge
and pump speeds for both aspiration and dispense. Separate speed buttons allow
individual control of both functions.
Matrix Technologies - www.matrixtechcorp.com
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