Summary of calibration tests and acceptance criteria
|Detector PDA or UVVis
||Measure max or maximum absorbance of anthracene solution (1 µg/mL).
||251 ± 3 nm 340 ± 3 nm
||Run pump at 1 mL/min (65% acetonitrile/water) and collect 10 mL from detector in a volumetric flask. Measure time.
||1.00 ± 0.05 mL/min
||Test all solvent lines at 2 mL/min with 0.1% acetone/ water, using step gradients of 10, 50, and 90%. Measure peak heights of respective step relative to 100% step.
||± 1% absolute
||Determine the peak area Relative Standard deviation (RSD) of ten 10-µL injections of ethylparaben (20 µg/mL).
||RSD < ± 0.5%
||Determine the coefficient of the linear correlation of one injection of 5, 10, 40, and 80 µL of ethylparaben solution.
||R > 0.999
||Determine the carryover of peak area by injecting 80 µL of mobile phase following an 80-µL injection of ethylparaben.
||Determine gravimetrically the average volume of water per injection withdrawn from a tared vial after six 50-µL injections.
||50 ± 2 mL
||Check actual column oven temperature with validated thermal probe.
||35 ± 2 °C
|* Waters Symmetry C18 (150 × 3.9 mm i.d., 5 µm)