May 11, 2009

Volume 87, Number 19 | p. 31 | First appeared online May 6

Chemical Ecology

Chemical Vital Signs

Living ants mask ever-present death cues with molecular indicators of vitality

Carmen Drahl

Video

Not Dead Yet

Argentine ant workers ignore pupae treated with a mixture of the "do not discard" chemicals dolichodial and iridomyrmecin.

Launch Video

Proc. Natl. Acad. Sci. USA * Macromedia Flash Player 8 is required to view video.

Proc. Natl. Acad. Sci. USA

BRING OUT YOUR DEAD An Argentine ant worker carries a pupa treated with extracts from dead ants to a garbage pile.

In "Monty Python and the Holy Grail," a film send-up of the Middle Ages and Arthurian legend, an elderly man resists being carted away from his village atop a pile of diseased corpses, loudly protesting, "I'm not dead yet." A new study suggests that ants have a chemical way of broadcasting that same information, which tells their nest mates not to toss them onto the rubbish heap.

Social insects such as ants carry dead colony members away from their nests to reduce the risk of disease. Traditionally, researchers have suggested that chemical decomposition products that accumulate after death stimulate ants to remove their former colleagues. But by studying Argentine ant workers, entomologists Dong-Hwan Choe, Jocelyn G. Millar, and Michael K. Rust of the University of California, Riverside, now provide evidence for an alternative explanation. They suggest that all ants, alive and dead, constantly carry chemical cues for disposal, but that live ants also possess molecular signals of vitality, which mask those of death (Proc. Natl. Acad. Sci. USA, DOI: 10.1073/pnas.0901270106).

By using thin-layer chromatography and gas chromatography to compare live, just-killed, and dead ants, the team showed that a subset of chemicals disappeared from the ants' outer covering within one hour of death. From those extracts they identified two rapidly dissipating "do not discard" signaling chemicals—specific isomers of the natural products dolichodial and iridomyrmecin.

ALIVE AND KICKING Argentine ants mask death signals by secreting a chemical cocktail that includes the compounds shown. Relative configurations of dolichodial (left) and iridomyrmecin (right) previously identified in Argentine ants are shown; absolute configurations remain unknown.

The team then rubbed ant pupae, which are immobile and have different chemicals on their outer coatings compared with adults, with both dolichodial and iridomyrmecin or with extracts from ants that were dead for at least one hour. They then dropped the pupae at the entrance to the nest. Normally, workers retrieve pupae and return them to the nest, but pupae treated with the do-not-discard chemicals were ignored without being carried until the chemicals dissipated, at which time the pupae were returned. Pupae treated with extracts from the dead ants were placed in the refuse pile. The team now hopes to apply their findings to develop pest-control strategies.

"Ants and other social insects are fascinating because they use a multilevel network of chemicals for communication," says Patrizia d'Ettorre, who studies communication in social insects at the University of Copenhagen. Although this exact signaling system may not be applicable to every ant species, the team's work is convincing because it combines behavioral and chemical studies, she adds.

Not Dead Yet

Argentine ant workers ignore pupae treated with a mixture of the "do not discard" chemicals dolichodial and iridomyrmecin.

Chemical & Engineering News

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