[an error occurred while processing this directive]
Skip to Main Content

Latest News

Advertise Here
September 8, 2010

Going The Distance

Structural Biology: Switchable fluorescence technique measures multiple distances in single molecules

Celia Arnaud

  • Print this article
  • Email the editor

Latest News

October 28, 2011

Speedy Homemade-Explosive Detector

Forensic Chemistry: A new method could increase the number of explosives detected by airport screeners.

Solar Panel Makers Cry Foul

Trade: U.S. companies complain of market dumping by China.

Novartis To Cut 2,000 Jobs

Layoffs follow similar moves by Amgen, AstraZeneca.

Nations Break Impasse On Waste

Environment: Ban to halt export of hazardous waste to developing world.

New Leader For Lawrence Livermore

Penrose (Parney) Albright will direct DOE national lab.

Hair Reveals Source Of People's Exposure To Mercury

Toxic Exposure: Mercury isotopes in human hair illuminate dietary and industrial sources.

Why The Long Fat?

Cancer Biochemistry: Mass spectrometry follows the metabolism of very long fatty acids in cancer cells.

Text Size A A

A new version of fluorescence resonance energy transfer (FRET) makes it possible to monitor multiple distances within individual molecules more easily than ever before (Nat. Meth., DOI: 10.1038/nmeth.1502).

Conventional FRET provides distance information because an acceptor chromophore fluoresces only when it is close enough to be excited by a nearby donor. Although FRET can be carried out with multiple acceptors, such experiments are difficult. By developing switchable FRET, Achillefs N. Kapanidis of the University of Oxford and coworkers there and at Bielefeld University, in Germany, avoid these complications by using acceptors that can be turned on and off with light. They can then install multiple copies of identical acceptors on individual biomolecules.

Using the cyanine dyes Cy3B and Cy5 as donor and acceptor, respectively, they monitor the structure of a DNA-protein complex and probe the conformational dynamics of a DNA Holliday junction—a structure formed from four strands of DNA—from two perspectives.

"This simplified switching method represents a significant and neat addition to the single-molecule toolbox and will nicely complement labeling and other advances in the multicolor single-molecule FRET field," says Ashok Deniz of Scripps Research Institute, one of the developers of three-color FRET.

Chemical & Engineering News
ISSN 0009-2347
Copyright © 2011 American Chemical Society
  • Print this article
  • Email the editor

Services & Tools

ACS Resources

ACS is the leading employment source for recruiting scientific professionals. ACS Careers and C&EN Classifieds provide employers direct access to scientific talent both in print and online. Jobseekers | Employers

» Join ACS

Join more than 161,000 professionals in the chemical sciences world-wide, as a member of the American Chemical Society.
» Join Now!