(now at Cytokinetics, South San Francisco), and coworkers reported the first use of ribosome display, a technique for synthesizing peptides that remain linked to their encoding mRNA. A major advantage of this technique is that it makes it easy to identify peptides of interest from a peptide library by reading their attached mRNA. And in 1997, two independent teams developed an mRNA-peptide fusion technique for hitching ribosome-synthesized peptides to their encoding mRNAs, with the antibiotic puromycin used as an intermediary. The groups were molecular biology professor Hiroshi Yanagawa, now at Keio University, Yokohama, Japan, and coworkers; and molecular biology professor Jack W.
by Stu Borman |
January 19, 2004