The evolved enzyme, called aMOx, is fairly productive, catalyzing about 3,800 oxidation reactions, or turnovers, before running out of steam, compared to fewer than 10 for earlier anti-Markovnikov oxidation catalysts. In conjunction with other reagents and catalysts, the enzyme could one day convert terminal-alkene substrates to a variety of aldehyde, alcohol, and amine products for commodity and fine chemical, pharmaceutical, and agrochemical use. The Caltech researchers started with an iron-based cytochrome P450 enzyme called P450LA1because it catalyzes a related reaction, metal-oxo alkene oxidation, in which a metal-linked oxygen attacks the double bond, typically forming an epoxide. An earlier study by another group had found that P450LA1 also made 19% aldehyde side-product by opening the epoxide.
by Stu Borman |
October 12, 2017