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Product Info Now October 2003
Plasmid prep service. Qiagen offers a plasmid
purification service that provides CGMP-grade ultrapure plasmid DNA in milligram
to gram amounts for commercial and clinical applications, including gene therapy
and genetic vaccination projects, and transfection into sensitive eukaryotic cell
lines. A range of extra options includes low- and ultralow-endotoxin grades and
restriction analysis. Proteomics system. The 4700 Proteomics
Discovery System directly links experimentally identified proteins to relevant
biological information and annotative tools of the Celera Discovery System online
platform, including direct links to biological and molecular function, SNP sites,
alternate splice forms, and comprehensive genetic information. Leveraging TOF/TOF
optics technology, the 4700 system helps researchers characterize proteins from
a variety of proteomics workflows, including gel-based and multidimensional liquid
chromatography (MDLC) experiments as well as the LC-MALDI approach. RNA protocol. The T7 protocol automates
all steps from isolating whole RNA to cRNA synthesis and purification. Yields
of cRNA produced with the automated protocol compare favorably to those obtained
from traditional manual methods run in tandem. Processed samples have proven to
hybridize successfully to Affymetrix GeneChips, yielding data fully comparable
to manual methods. The new protocol is a step in amplifying RNA generated from
small amounts of sample materials for DNA microarray experiments.
NMR software. The Delta software program
combines spectrometer control and data management into a single robust application.
Operating in both Windows and Linux environments, the program allows users to
perform tasks typically accomplished through the use of multiple programs. Output
can be displayed in a variety of formats, including PostScript, JPEG, PDF, and
encapsulated PostScript.
Genotyping system. The GenomeLab system
includes GenomeLab SNPstream, capable of performing 4600 to over 800,000 genotypes
per day, and the CEQ 8800 Genetic Analysis System, which processes two 96-well
plates and tracks the samples. The CEQ 8800’s genetic analysis applications including
DNA sequencing, allele identification, amplified fragment-length polymorphism
fingerprinting, SNP scoring, and heterozygote detection.
Bioreactors. The Cell Optimizer and Mini-Pilot
Plant systems feature automated control over a range of culture volumes from 250
mL to 45 L. The bioreactors can grow a variety of cell lines, including mammalian,
plant, insect, bacteria, and fungi. The systems provide monitoring and control
of pH, dissolved oxygen, and temperature. Individual components can be added for
scale-up processes. Disposable tips. An option for the Quad-Z
215 Liquid Handler, the addition of the tips to the handling unit increases throughput,
efficiency, and reproducibility. The autoclavable tips have a dispensing volume
range of 1-200 µL. The handler can also be configured with fixed probes for applications
that do not require disposable tips. SPE columns. An information data sheet
describes the new Microparticulate Cerex SPE columns. The columns use microparticulate
packing that requires smaller sorbent beds than equivalent conventional SPE packings.
The patented Cerex packing process allows for very short bed paths without channeling,
which reduces back pressure. Sample preparation kits and reagents. The
products in the Ettan sample prep range are the Albumin and IgG Removal Kit, 2-D
Fractionation Kit, and basic chemicals and reagents. These kits and reagents remove
contaminating compounds. They are used to prepare samples for 2-D electrophoresis,
mass spectrometry, and Western blotting. Plasmid mini-kit. The PerfectPrep model
enables the isolation of ultrapure plasmid DNA within 20 min. Automated sequencing
produces extremely long read lengths with consistently low error rates. High reproducible
yields are obtained via an enhanced alkaline lysis step coupled with a specially
activated silica matrix. DNA purification. The Two-Step Affinity
Purification System provides a complete standardized solution for the purification
of ultrapure (>98% pure) full-length recombinant proteins, even from eukaryotic
cell lysates. Ultrahigh purity is achieved by sequential affinity purification
via two small affinity tags, the 6xHis and Strep-tag. The complete system
includes expression vectors, purification matrices, and antibodies.
Antibodies. These new offerings are specific
for acetylated and phosphorylated human p53 as well as WIP1 (PPM1D), a serine/threonine
phosphatase that is a transcriptional target of p53 and is implicated in cell
cycle control, spermatogenesis, and lymphoid cell function.
Ion trap. The 4000 Q Trap LC/ MS/ MS system
provides MS/MS capabilities to improve workflow, deliver enhanced data quality,
and be up to 10 times as sensitive. The 4000 Q Trap combines high-sensitivity
triple-quadrupole technology with the high-performance features of an ion trap
for both qualitative and quantitative capabilities for protein and small-molecule
research. HPLC. The cLC system uses various
modes of detection, including UV/VIS, ELSD, MS, and RI. The multifunctional system
incorporates preparative, analytical, and radial compression columns. Up to 15
g of synthetic organic compounds can be purified per run, and the system is outfitted
to accommodate multiple columns with both polar and nonpolar solvents.
Separation system. The Carr Powerfuge Pilot
expedites the development of liquid/solid separations prior to full process scale-up.
Flow rates from 0.1-4 L/min and RCFs from 500 to 20,000g can be used for
applications such as fermentation, vaccine production, cell harvesting, and cell
debris clarification. Sealed feed and drain lines provide aerosol-free operation,
and dry compacted solids can be removed without special tools. Brochure. This four-color brochure details
the benefits of the Cerex System-48 multiple SPE column processor and includes
pictures of the unit, specifications, and information on compatible columns. The
unit uses positive pressure rather than vacuum to process up to 48 1- to 6-mL
columns simultaneously. Labeling kit. The CyDye DIGE Fluor Labeling
Kit is designed for scarce samples and enables labeling and comparison of as little
as 5 µg of material. It allows customers to use 2-D DIGE methodology for the analysis
of minute protein abundance differences. The kit includes two different dyes. Genetic analyzers. The Prism series uses
capillary electrophoresis technology to provide the appropriate genetic analysis
for the laboratory. The 310 is a single-capillary analyzer for academic and forensic
laboratories. The 3100 Avant expands on the 310’s capability with a 4-capillary
system to provide sophisticated automation capabilities. The 3100 is an automated
16-capillary system that delivers accurate, reliable results across the complete
range of sequencing and genotyping applications. Processing system. The Syncore uses a modular
concept to increase productivity in parallel and combinatorial chemistry. The
system can run from 4 to 96 samples simultaneously, handling volumes up to 500
mL. Bumping is eliminated with an adjustable vortex eccentricity. Available accessories
include the Polyvap parallel evaporator, which performs multiple evaporations
of different volumes, and the Syncore analyst, which provides parallel evaporation
for residue analysis.
Newsletter. This quarterly Web-based newsletter
provides news in bioinformatics and updates on solutions for the life science
industry. Tissue cultures. The BIO 101 series is
a family of plant tissue culture media that include Murashige and Skoog, Gamborg’s
B5, and Chu’s N6. Vitamin mix, basal salts, basal medium, complete medium, and
complete medium with agar are offered for each growth formulation. Website. This site gives life scientists
access to a comprehensive range of application notes, product overviews, seminars,
oral and poster presentations, and papers. Application- focused sections enable
researchers to readily find information on proteomics, genomics, small molecules,
biomolecules, sample preparation, electrophoresis, and fundamentals such as MALDI-TOF
MS and MS/MS fragmentation.
Peptide and protein biomarker. This automated
sample preparation system is based on magnetic-bead biological recognition technology.
The ClinProt selectively captures and enriches certain classes of proteins and
peptides contiguously from serum or other biological samples. It is configured
to be used with a 96-well plate for reliable throughput and reproducibility. Handbook. An applications handbook and
catalog, this 744-page publication includes technical sections on protein/gene
expression, protein purification, other protein-related products, gas chromatography
and reagents, and antibody production and related products. PCR instrument. The COBAS TaqMan 48 analyzer
is an in vitro diagnostic (IVD) device combining IVD and real-time PCR capabilities
in one instrument. The TaqMan provides researchers with a fully automated tool
for tissue and cell disruption. It features real-time quantitation and can increase
output, having eliminated a separate detection step. Compounds. A 96- or 384-well plate can
be customized with target sets of compounds that are at least 90% pure and can
be used for HTS. The four libraries feature over 250,000 compounds that can be
tailored to researchers’ specifications, such as ADME properties, specific amounts,
and diversity criteria. Dye kits. CyDye fluors are specifically
formatted for labeling proteins or antibodies in protein array experiments. Each
kit contains two vials of protein-array-grade monoreactive dye packaged in a color-coded
Securitainer for ease of identification. The kits, one containing Cy3 monoreactive
dye and the other Cy5, allow researchers to perform either one-color sandwich
assays or multiplexed differential analysis. The kits contain sufficient dye to
label material for 20 to 80 protein array slides.
Pump. Used for proteomics applications,
the 1100 series nanoflow liquid chromatography (LC) system for MS includes a nanoflow
pump with electronic flow control. This system provides stable, ultralow-flow
control for a constant level of high sensitivity and reproducibility for protein
analysis. Software. A chemically intelligent decision
support system for drug discovery project scientists, Decision Manager lets scientists
assemble and share data from many sources; analyze data visually using property-based,
structure-based, statistical, and chemical rules sets; and apply analysis results
as new queries against data repositories to find similar compounds. Product guide. This full-color guide describes
reagents for B-cell signaling studies, including antibodies, ligands, receptors,
and ELISA kits for CD40L, BAFF, and APRIL. |
Freezer.
Using advanced composite thin-wall insulation to maximize internal volume, the
Harris Platinum SI Series -85 °C freezers hold up to 30% more inventory than conventional
ultralow-temperature freezers of equivalent size. Incorporating an RS485 data
port for two-way communication, the freezer uses CryoSoft software for remote
control and monitoring of temperature, set points, and freezer performance.
DNA
isolation. The Ultrafree-DA centrifugal device facilitates the single-step
recovery of 100- to 10,000-base-pair DNA fragments from agarose gel slices. A
band of interest is placed into the preassembled filter device, and centrifugal
force extrudes the DNA from the gel pores and into a collection tube, where it
is ready for sequencing, cloning, or labeling.
Peptide
synthesizers. The Odyssey System of microwave-enhanced peptide synthesis
is 10-20 times as fast as traditional methods and produces cleaner peptides with
higher yields. The line includes manual units and automated systems that can synthesize
up to 12 different peptides. The systems perform automated resin cleavage and
synthesize peptides throughout a wide scale, from 0.05 to 5 mmol. The Odyssey’s
compact design conserves bench space and fits in a fume hood.
Chromatography.
MDS Sciex and LEAP Technologies are introducing the 1D NanoLC System, an advanced
automation package that delivers turnkey solutions for nanoLC. It is the first
HPLC system to allow nanoscale LC/MS without flow splitting. By incorporating
innovative direct pumping technology, the 1D NanoLC System achieves unsurpassed
sensitivity, reliability, and stability. Its automated peak-parking feature lowers
the flow rate rapidly during a gradient, eliminating previously complex approaches
and saving time.
Capsule.
The Minimate Tangential Flow Filtration Capsule is a disposable filtration device
for research and entry-level production of new drugs. The filtration capsule enables
rapid prototyping to accelerate and simplify scale-up for many bioprocessing applications.
It is designed for single use, eliminating the need for cleaning and cleaning
validation. With a range of 10 mL to 2 L, it can be used in all areas of bioprocessing.
Furnace.
The AAS ZEEnit 65 has a deuterium background compensation (D2 BGC).
This graphite furnace can be operated with background correction technique, and
it allows hydride AAS with D2 BGC. The system allows the analysis of
high element concentrations, and the three-field mode allows higher contents to
be analyzed without dilution.
Scanner.
The 2DCYPHER system offers a complete solution for high-throughput, individual
sample identification and tracking. It individually codes cluster tubes with a
14 x 14 array to produce more than 3.6 x 1015 unique codes. In addition,
a human-readable, 10-digit alphanumeric code around the data matrix facilitates
manual tube identification. Options include 0.65- and 1.2-mL tube formats, lockable
racks, and sealing options such as silicone plugs, caps, and septum plugs.
RNA
reagent. Based on a lipid formulation, RNAiFect gives efficient transfection
of siRNA into a wide range of eukaryotic cells. RNAiFect provides high transfection
efficiency with low cytotoxicity. Its low endotoxin levels and testing for the
absence of RNase activity ensure reproducible performance in the most demanding
RNAi experiments. It can be used for transfecting a wide range of cell types,
including AGS, HEK-293, HeLaS3, Huh-7, HUVEC, NIH-3T3, and PA317 cells.
Water
purifiers. The AFS-8 and AFS-16 analyzer feed systems are designed
to produce reagent water from tap water for direct feed to clinical analyzers.
Both systems use activated carbon, particulate prefiltration, and ion-exhange
resins to produce Type I water. The AFS systems have Class One Medical Device
status (under FDA citation 21 CFR 862.2050), and the water generated by
the systems meets NCCLS (National Committee for Clinical Laboratory Standards)
Type I and Type II reagent water specifications.
Normalization
kits. GAPDH whole-cell normalization kits provide a sensitive and accurate
method for protein normalization in a ready-to-use format. The kits are designed
to reduce inaccuracies in experiments and provide more meaningful results. Each
kit is supplied in a convenient 96-strip well format, which allows the user to
process multiple samples quickly at any level of throughput.
Filter
plates. MultiScreen-MIC 96-well filter plates are a high-throughput
tool designed for testing leads in new drug development efforts. They are optimized
for migration, invasion, and chemotaxis assays. These versatile systems incorporate
3-, 5-, or 8-µm polycarbonate membranes for use with a variety of cell types and
are QC-released to support both adherent and suspension cell lines.
Pipetting
system. The Mosquito system can pipet accurately and reproducibly through
the 50-nL to 1.2-µL volumetric range, producing CVs of <10% at 50 nL. It has
a reel of 35,000 micropipets and disposable tips that eliminate cross-contamination.
The Mosquito is ideal for subcloning plates during compound dissolution in 96-,
384-, and 1536-well plates.