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Product Info Now: March 2004 Featured Products: Scavenger resins One modern technique for purifying reactions in drug discovery is the use of polymer-bound scavenger reagents. Two new palladium scavenger resins have been developed with which greater than 99% removal of palladium has been demonstrated by simply stirring either of them with a solution of the compound of interest at room temperature for 4–24 h. A typical scavenging procedure is to add 2.2 molar equivalents of either PS-2-mercaptonicotinamide (1) or PS-dithiothreitol (2) to the target molecule dissolved in a tetrahydrofuran (THF) solution. The suspension is either agitated or stirred for 4–24 h and then filtered. The polymer reagent is washed twice with THF and filtered. The combined THF filtrates are then concentrated in vacuo to yield the final product. Test reactions used 22 mg of palladium acetate [Pd(OAc)2] and 220 mg of scavenging resin. After 4–24 h of agitation, less than 1 mg of mass was recovered. When tetrakis(triphenylphosphine)palladium(0) [Pd(Ph3P)4] was used as the palladium source, only triphenylphosphine [Ph3P] was recovered as determined by 1H-NMR spectroscopy and elemental analysis. Sigma-Aldrich - www.sigmaaldrich.com More Products: Assay platform. HALO is a rapid, high-throughput,
multifunctional hemotoxicity testing system that can be used at all stages of
drug development, from screening to clinical trials. Based on the classical semisolid
colony-forming procedure, the system is a proliferation assay with nonsubjective
luminescence readout. HALO is a standardized assay performed in 96-well plates.
The HALO Kit Platform detects and measures at least 14 different hematopoietic
populations either individually or in combination for 5 different species. The
kits are available in different sizes for maximum flexibility to perform any in
vitro hema topoietic/ hemotoxicity testing. Workstation. The Eppendorf epMotion 5070
liquid-handling workstation enables rapid and reproducible liquid handling through
automated pipetting, dispensing, and dispensing variants. Pipetting sequences
and protocols previously performed manually can be transferred to the epMotion
5070, which is controlled via the mouse and keyboard of the integrated control
panel instead of a PC. An optical sensor system controls and ensures the reliability
of the procedure. RNA isolation. With tissue samples for
genomic analysis often small and irreplaceable, an RNA isolation service helps
ensure maximum RNA recovery and high-quality gene expression data. GenUs BioSystems’
bioarray service laboratory offers researchers an alternative to performing this
delicate step in the gene expression profiling process in their own laboratory.
Established and proven RNA isolation methods take small amounts of cell and tissue
samples and generate gene expression data on which researchers can draw sound
conclusions. Chromatography medium. For optimized purification
of His-tagged proteins, the Ni Sepharose High Performance affinity chromatography
medium provides better protein binding capacity, negligible Ni leakage, and compatibility
with a wide range of reducing agents, detergents, denaturants, and other additives.
Its 34-µm bead matrix offers the highest binding capacity available to provide
more-concentrated samples, higher efficiency, time savings, and increased yield. Centrifuges. The Allegra X-12 Series benchtop
centrifuges deliver unique capabilities that streamline cell separations. The
X-12R and X-12 spin up to 4 x 750 mL and feature new cell culture flask adapters
to enable direct centrifugation of cell culture flasks. The X-12 Series incorporates
ARIES Smart Balance rotor technology, which automatically corrects imbalance and
continues the run without shutdown. Standard and ARIES versions of the X-12 Series
4 x 750 mL swinging bucket rotor are available. Gel purification. This system combines
nontoxic hydrogel electrophoresis with a revolutionary elution technology, resulting
in higher yields and better quality than PAGE (polyacrylamide gel electrophoresis,
until now the standard gel purification technology). The new technology also provides
the advantages of faster turnaround time, quality control by MALDI TOF mass spectrometry
analysis for all oligos up to 120-mer, and gel slice excision in blue light. This
gel purification system is available for unmodified oligonucleotides of all lengths
up to 150 bases. Catalog. The 2004 Scienceware edition features
pipettors and a range of sizes and styles of desiccators suitable for all laboratory
requirements. There are also safety-related products for protection from laboratory
hazards and new racks for tubes, dishes, vessels, and instruments. Every laboratory,
production facility, and educational setting will find many useful aids and standard
science items in this catalog. To receive a copy, call toll-free at 800-423-5278
or visit online. HPLC mobile phases. CHEM+ NECT packaging
technology provides a disposable, closed-system alternative to the traditional
and potentially hazardous preparation of HPLC mobile phases. These premixed mobile
phases are manufactured to customer specifications using reagent-grade components
to ensure equivalent performance, and they can be easily connected to any HPLC
system. CHEM+NECT solutions are also offered with stability data to validate long-term
consistency. Screening system. The LabChip 3000 Drug
Discovery System performs unattended biochemical and cell-based screening assays
using Caliper’s microfluidic chips. The company notes that this addition
to its suite of LabChip microfluidic systems is the first major product introduction
since Zymark Corp. and Caliper Technologies combined to create Caliper Life Sciences.
The LabChip 3000 system is an updated, much smaller, more affordable version of
the Caliper 250 system. Plasma analysis. The Plasma Metanephrine
Analysis Kit extracts free metanephrine (MN) and normetanephrine (NMN) from plasma
for HPLC–electrochemical detection analysis of the catecholamines’ metabolites.
The kit offers more direct correlation of plasma free metanephrine concentration
to the catecholamine and metanephrine production of adrenomedullary and pheochromocytoma
cells than other sources. Its method detects all but the smallest of pheochromocytomas,
yielding fewer false positive results than traditional biochemical tests. PCR kits. The Knockout RNAi Clone &
Confirm PCR Kits provide a simple and efficient way to generate candidate small-hairpin
(shRNA) expression cassettes (SECs) precloned into vectors in as little as 4.5
h. The method is faster than those of other PCR-based RNAi kits. Unlike other
PCR-based systems, users need only a single round of PCR to generate a SEC suitable
for functional testing. Users also can propagate and study only those shRNA clones
whose corresponding SECs proved effective in the initial experiment. Particle size analyzers. The Insitec is
the only fully compliant on-line analyzer that makes use of industry-standard
laser diffraction particle size analysis. It is therefore uniquely placed to serve
the process analytical technology needs of pharmaceutical milling, micronization,
spray drying, compaction, and granulation manufacturers looking to tighten quality
control, minimize off-specification material, and gain greater insight into individual
processes. Pipette tips. ART (aerosol-resistant tip)
pipette tips contain a patented barrier, are scientifically proven to eliminate
carryover contamination, and are suitable for PCR procedures and handling radioactive
and highly infectious samples. The barrier material is nonwicking, self-sealing,
hydrophobic HDPE that effectively blocks all liquid and aerosol passage. In addition,
the tips are certified to be RNase- and DNase-free by independent laboratories. Sample processor. The Tissue Lyser system
provides high-throughput processing for simultaneous, rapid, and effective disruption
of up to 192 biological samples, including animal and plant tissues, bacteria,
and yeast. More than 20 optimized disruption and homogenization protocols integrate
the TissueLyser with other systems for purification of high-performance nucleic
acids from a wide range of sample types. The system is easily scalable from single
samples up to 2 x 96 samples in parallel. Highly reproducible processing of up
to 192 samples takes as little as 2–4 min. Catalog. Polysciences’ 2004–2005
edition lists more than 3000 off-the-shelf products used by laboratory technicians,
chemists, scientists, and engineers in histology, microscopy, polymer chemistry,
and life sciences. These products include fine chemicals, certified and fluorescent
dyes, electrophoresis reagents, magnetic and nonmagnetic particles, particle kits,
microspheres, monomers, and polymers. The catalog lists all relevant technical
data on each product as well as hazard, handling, and storage codes. Catalog. The Bionet Research Intermediates
Catalog for 2004–2005 showcases almost 2300 building blocks, including some
new compounds resulting from the recent acquisition of Merlin synthesis. The catalog
is multiple-indexed to suit everyone’s preferred search style, and a CD-ROM
containing more than 40,000 unique and varied screening compounds is supplied
with each copy. Sample processors. The Metrohm USB line
offers unprecedented power and flexibility in the automation of Metrohm Titrandos.
Model 814 is ideal for analyzing small to medium numbers of samples, and Model
815 Robotic Sample Processor XL is available for large numbers. Applications include
full automation of direct-inject Karl Fischer titrations, potentiometric titrations,
and environmental samples. Combining a Titrando and a USB sample processor offers
security and traceability—and is ideal for today’s GMP/21 CFR
Part 11 laboratory. Membrane filters. Supor EKV 0.2 micron
sterilizing-grade filters are designed for economical filtration of buffers, biologicals,
tissue culture media, ophthalmic products, and many other biopharmaceutical fluids.
These unique poly(ether sulfone) (PES) membrane filters contain a built-in prefilter
layer that has been developed with a proprietary, asymmetrical, V-shaped, funnel-like
pore structure that increases throughput to extend filter life and allows for
economical design of filtration systems. Supor EKV filters will be on display
at INTERPHEX 2004, March 16–18, in New York, Booth 1321. Online platform. The Celera Discovery System
online platform is a subscription-based, integrated research platform that provides
online access to the most comprehensive and current set of curated biological
data and analysis tools available for comparing human and mouse genomes. The platform
includes an interactive, customizable Map Viewer that provides graphical displays
for comparing human and mouse genes within their genomic contexts. Autosampler. The Sample Sentinel refrigerated
unit offers robotic liquid-chromatographic autosampling by moving the sample vial
to sampling, diluting, or mixing stations without requiring complex mechanical
drivers. Capable of transferring 105 vials (300 µL or 1.8 mL) in three removable
trays when cooled or 120 vials when uncooled, the unit is designed for use with
microbore, standard-bore, or preparative applications and is available for precise
fixed-loop and variable-loop injection. With the sample preparation option, up
to three reagent additions are programmable in microliter volumes with virtually
unlimited programmable steps. Reagents. The Qtracker 655 Kit is designed
for loading Qdot nanocrystals into live cells. The kit uses custom targeting peptides
to deliver highly luminescent Qdot quantum dots to the cytoplasm. Once inside
the cells, Qtracker labels provide strong, stable fluorescence that does not bleach
over time, even after extensive continuous illumination and observation. Qtracker
quantum dots add no additional autofluorescence, are not transferred to adjacent
cells in the population, and display excellent retention properties; the nanocrystals
used in the kit remain inert and nontoxic to many cell types and can be followed
in some cell lines for up to a week. Reagents. Brilliant SYBR Green-based QRT-PCR
reagents provide a universal solution to real-time PCR detection and gene quantification.
The SYBR Green dye binds specifically to double-stranded PCR products without
the need for sequence-specific probes. The QRT-PCR reagents are optimized on Stratagene’s
Mx4000 multiplex quantitative PCR system and Mx3000 real-time PCR system, and
are validated on most other QPCR instruments. Software. The ActiveSite software add-on
to the BioCAChe, BioMed-CAChe, and WorkSystem Pro products provides powerful tools
to speed the drug discovery process by allowing researchers to predict and select
better-quality leads before making them in the laboratory. Scientists can import
and display 3D proteins directly from the Protein Data Bank and then view, analyze,
and modify them by using interactive sequence and 3D structure editor windows.
Users also can optimize proteins, dock ligands, and model reactions of molecules
with tens of thousands of atoms. Dispenser. The WellMate is a high-speed,
small-footprint, 8-channel fluid dispenser for 96- and 384-well microplates. It
features a height-adjustable dispense head to accommodate dispensing to shallow-
and deep-well plates and blocks; low-cost, disposable, presterilized tubing cartridges
that enable dispense volumes of 2.0–2000 µL; programmability in 1.0-µL
increments; memory for storing as many as 18 dispense protocols; and a digital
keypad. RS232 programming promotes robotic integration, and a removable plate
stage makes cleaning fast and easy. Filter plates. The one-piece design of
MultiScreenHTS filter plates, specifically developed for automated workstations,
features rigid sidewalls for robotic gripper arms, providing ample surface to
accommodate bar-code labels. In addition, a removable underdrain allows easy membrane
access. The high-throughput plates are optimized for general sample preparation,
ELISpot, whole-organism visualization, and radioisotope detection applications.
MultiScreenHTS filter plates are available in a range of plastics and membranes. PCR system. The FailSafe Real-Time PCR
System incorporates SYBR Green I dye for detecting double-stranded DNA generated
during PCR. During each phase of DNA synthesis, the SYBR Green I dye binds to
the amplified PCR products, and the amplicon quantity is monitored in real time
by measuring its fluorescence. The incorporation of SYBR Green I dye into a real-time
PCR reaction provides great flexibility because no target-specific probes are
required. |
ISSN 1532-4486
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