These normalized cDNA library construction services are produced from customers’
custom cDNA libraries (i.e., standard libraries, microquantity libraries, etc.).
ExTREme technology enables greater numbers and lengths of high-quality clones
to be generated in less time while substantially reducing abundant genes.
Biotinylated driver RNA and single-stranded (ss) target DNA from the cDNA library
are hybridized to each other at a low Cot value (concentration of driver times
the time of hybridization) in accordance with the ExTREme protocol. Undesired
hybrids are removed by phenol extraction; the ss target DNA is converted to double-stranded
DNA and transformed into T1 phage-resistant E. coli. This process reduces
the level of abundant and moderately abundant genes and enriches for previously
undiscovered rare genes.
ExTREme technology typically enables libraries of 10 X 106 primary
clones with >96% recombinant clones containing >1.5-kb average insert size
to be produced. ExTREme technology also enables delivery of normalized libraries
in less time, 7 to 10 weeks, compared with the typical 10 to 12 weeks.
Also offered are standard, nanoquantity, and subtracted cDNA libraries as well
as total RNA purification from tissue, mRNA purification from tissue, mRNA purification
from total RNA, and cDNA library amplification. All libraries feature directionally
cloned cDNA for expression, antibody, screening, subtraction, and normalization. Express Genomics - www.express-genomics.com
Solid
reagent option for microwave flow reactor
The Voyager Microwave Flow-Through Synthesis System offers options for handling
solids, slurries, and highly viscous fluids. Two separate flow-through modules
are designed to handle the entire range of scale-up applications.
The Voyager CF (Continuous Flow) module is the choice for scaling up solution-
based chemistries, while the Voyager SF (Stop Flow) module (pictured above) is
the option for scaling up chemistries with solid reagents, highly viscous materials,
or insoluble products. Both modules allow the automated scale-up of chemistries
with microwave energy, all with a completely safe, selfmonitored platform. The
SF option allows for the synthesis of up to 50 mL of material automatically, giving
chemists all of the safety advantages of the Voyager flow-through design while
yielding the productivity and solids compatibility found with static batch systems.
As part of the Discover family of microwave synthesis products, Voyager can
handle single, small-scale reactions as well as scale-up to kilogram quantities.
The system also can perform atmospheric and pressurized reactions, and it has
temperature and pressure feedback control.
The use of active flow cells allows the user to suspend reagent beds in the
flow stream. These solid reagent beds can consist of immobilized reagents, catalysts,
scavengers, or coupling enhancers. Active flow cells can be prepared by the user
to allow maximum versatility in the process.
The 845Zprep System improves injection speed and carryover, and reduces maintenance
issues from injection ports for semipreparative to preparative autopurification
applications. The system's Injection Module features a stainless steel, 2-position,
6-port valve with 0.040-in. (1.0-mm) port diameters to accommodate flow rates
from 5 to 150 mL/min. The Injection Module aspirates the sample directly into
the sample loop for faster injection cycling time while minimizing carryover.
The 845Zprep also uses a Low-Mount Fraction Collection Valve for separate injection
and fraction collection fluid paths. Gilson - www.gilson.com
Neuropeptide antibodies.
Antibodies to the neuropeptides Rab3a, synaptosomal-associated protein of 25 kDa
(SNAP25), synapsin, and synuclein are available. These antibodies complement other
neuropeptide antibodies such as β-amyloid,
γ-γ
enolase, neuron-specific enolase, glial fibrillary acidic protein (GFAP), neurofilament,
tangles, and PHF-tau. Pierce Biotechnology/Endogen - www.endogen.com
Protein
purification.
EasyLyse Bacterial Protein Extraction Solutionis designed to gently purify
proteins, especially recombinant proteins expressed in E. coli, so that
they retain more enzymatic activity. Formulated as a homogeneous reagent, EasyLyse
is convenient for high-throughput applications. Simply combine the Enzyme Mix
with the Lysis Buffer and add to cells for rapid, one-step lysis and removal of
viscosity. EasyLyse contains a lytic enzyme with high specific activity, a nuclease
to reduce viscosity, and a nondenaturing detergent. Epicentre - www.epicentre.com/easylyse.asp
Cycloamylose refolding.
The Refolding CA Kit provides a two-step procedure that optimizes the refolding
conditions of inclusion body proteins. This optimization results in a high refolding
efficiency for restoration of protein activity. The kit is supplied with guanidine
hydrochloride and DTT for protein unfolding, four different surfactants that can
be added independently to the unfolded protein solution to provide protection
against molecular aggregation, and highly polymerized cycloamylose for surfactant
removal and recovery of protein activity. Reagents are provided for 25 refolding
reactions (0.24 mg of protein per reaction). Takara Mirus Bio - www.takara.com
Software.
Varia is designed for researchers involved in high-volume genetic analysis. Features
include navigable displays of the complete human genome at the nucleotide scale,
algorithms for identifying regions of autozygosity, automated tools for building
haplotype maps and constructing pedigrees from genotype data, and linkage analysis
tools for the assocation of variation-to-variation or variation-to-disease. Silicon Genetics - www.silicongenetics.com
Monoclonal antibodies.
NeoClone uses a proprietary process to generate high-affinity monoclonal antibodies
(mAbs) more rapidly and less expensively than traditional hybridoma methods. The
service typically includes generation of 20 mAbs against your antigen; you own
the cell lines as well. Additionally, because the mAb-producing cell line is diploid,
the time and cost to develop additional antibody are greatly reduced. NeoClone
Biotechnology - www.neoclone.com
Discovery
system.
The LabChip 3000 Drug Discovery System is approximately one-third the size of
a Caliper 250 system and more affordably priced. The LabChip 3000 runs assays
that cover the most frequently screened target classes, including kinases, phosphatases,
proteases, and GPCRs. Increasingly, such assays are being applied not only in
primary screening but throughout the drug discovery process, including selectivity
screening and generation of structure–activity relationships (SARs). Caliper Life Sciences - www.caliperLS.com
Catalog.
The 2003/2004 Organics, Inorganics, Metals, and Materials Catalog includes
more than 1200 new products and the entire range of Avocado Organic compounds.
This edition carries over 20,000 items, including organic compounds, high-purity
inorganics, pure metals, alloys, elements, precious metal compounds and catalysts,
rare earths, AA/ICP standards, precious metal labware, and more. For a free copy,
call 1-800-343-0660 or 1-978-521-6300 (outside the U.S.). Alfa Aesar -
www.alfa.com
Purification kit.
The BACMAX DNA kit was developed to isolate high-quality BAC DNA. The scalable
protocol is based on a modified alkaline lysis procedure that typically yields
0.6 to 25 µg of BAC DNA from 1.5- to 100-mL cultures of a single-copy BAC. Selective
precipitation steps and the incorporation of the RiboShredder RNase Blend effectively
remove contaminants that degrade DNA and interfere with downstream applications.
There is no need for columns, resins, or organic extractions. Epicentre
- www.epicentre.com/bacmax.asp
Antioxidant
assay.
Designed to measure the overall antioxidant capacity in a given sample, this assay
from Cayman Chemical relies on the ability of antioxidants in the sample to inhibit
the oxidation of ABTS in comparison to Trolox, a water-soluble tocopherol analogue.
By quantifying the cumulative effect of all antioxidants present, more relevant
biological information is acquired. The assay comes in a convenient 96-well plate
format for a variety of sample types, including plasma, serum, urine, saliva,
and cell lysates. Cayman Chemical - www.caymanchem.com
Brochure.
This six-page color brochure features the AniGARD II Animal Transfer Station,
a vertical-flow recirculating-air clean bench designed to provide animal protection
and particulate control during routine cage-changing operations in the laboratory
while maximizing productivity and flexibility. The AniGARD II permits operator
access on three sides while providing ISO Class 5 (Class 100) protection by delivering
HEPA-filtered air to the work surface. This lightweight, easily maneuverable cabinet
features an ergonomically engineered, adjustable work surface and slanted view-screen
for increased worker comfort. Baker Co. - www.bakerco.com
Platform.
The Certified ArrayScan Platform is equipped with an integrated solution featuring
a preowned Certified ArrayScan HCS Reader equipped with a suite of BioApplications
image analysis algorithms for comprehensive multiparametric assay capability.
Rich cellular data is managed automatically by a complete range of HCS informatics
tools. The Certified ArrayScan promotion is a low-cost solution for accessing
high-content biology. Cellomics - www.cellomics.com
Cytokine arrays.
The ProteoPlex 16-Well Human Cytokine Array Kit provides a complete solution for
determining the levels of 12 cytokines from 10 sera or tissue culture supernatants
simultaneously. Conventional cytokine measurements are based on single-analyte
detection, requiring multiple kits and large sample volumes to test for multiple
cytokines. In contrast, the ProteoPlex arrays require only 50 µL of the sample
material to determine concentrations of 12 different cytokines. EMD Biosciences
- Novagen - www.emdbiosciences.com
Evaporator.
The EZ-2's patented sample protection systems prevent bumping and overheating
of delicate samples while ensuring smooth, fast evaporation of the solvent, even
with difficult mixtures. The system accommodates a wide selection of sample holders,
enabling evaporation from most common sample container formats. The EZ-2 is designed
to take tubes, flasks, and vials directly from the synthesis process, eliminating
a manual handling step, increasing recoveries, and removing the chance of cross-contamination. Genevac - www.genevac.com
Protein.
BD Matrigel Matrix, High Concentration (HC), is now available from BD Biosciences
Discovery Labware. BD Matrigel Matrix is a solubilized basement membrane preparation
extracted from EHS mouse sarcoma, a tumor rich in ECM proteins. Its major component
is laminin, followed by collagen IV, heparan sulfate proteoglycans, and entactin.
BD Matrigel Matrix, HC,is used for in vivo angiogenesis assays and to
augment the growth of human tumors in nude mice. BD Biosciences - www.bdbiosciences.com
Liquid-handling system.
For cell-based assays including ELISA, ADME/Tox, plasmid prep, and RNA/DNA isolation,
the TekBench liquid-handling system contains storage for over 1000 microplates,
containers, and consumables (equal to 5 carousels). Standard options include UV
sterilization, HEPA filter, chilled reagent storage, and nanoliter to microliter
pipetting tools with wash stations. The working deck supports a variety of third-party
devices, including readers, plate washers, bulk dispensers, filtration manifolds,
and shakers. TekCel - www.tekcel.com
Bioreactor.
The miniPERM benchtop bioreactor consists of nutrient and production modules separated
by a semipermeable membrane. Cells and cell products such as antibodies and other
high-MWCO components are retained in the production module, resulting in high
concentrations of product for easy harvest and purification. The dialysis membrane
facilitates exchange of oxygen, CO2, nutrients, and waste for optimal
growth and production conditions. Greiner Bio-One - www.gbo.com/bioscience
Microplate
cytometer.
The Acumen Explorer 405-nm model enables the β-lactamase
and blue fluorescent protein reporter systems that, until now, have routinely
been measured by bulk fluorescence readers as single-point readouts. In contrast,
this model offers multiparametric analysis at high throughput, allowing rapid,
high-content assays. Acumen Explorer 405 provides greater assay sensitivity on
plate formats ranging from 96-well to the 1536- and 3456-well microplates used
in µHTS. TTP Labtech - www.ttplabtech.com
Brochure.
This publication features a wide variety of polymer-supported reagents and scavengers
for solution-phase synthesis. Included are electrophile, nucleophile, and metal
scavengers, polymer-supported reagents, and polymer-supported catalysts. E-mail
drugdiscovery@sial.com to receive
free copy. Sigma-Aldrich - www.sigma-aldrich.com
Confocal imaging.
Opera is a new generation of confocal microplate imaging readers providing solutions
for fully automated high-speed and high-resolution screening. The Opera has a
dispensing unit available and extends the application portfolio to cellular kinetics.
The dispenser delivers 0.5 to 15 µL of a reagent to a well of a 96-, 384-, or
1536-well plate in less than 1 s (CV < 5%). The liquid is injected into the
samples as small droplets, which ensures fast mixing. The reagent reservoir can
be cooled to 4 °C. Evotec Technologies - www.evotec-technologies.com
Chromatograph.
The ACQUITY ultraperformance LC system uses patented 1.7-µm hybrid particles within
the columns to obtain faster run times than HPLC systems. The 1.7-µm particle
is a pressure-tolerant, reverse-phase particle made of organic (e.g., organosiloxane)
and inorganic (silica) elements. Each column features an eCord that records the
number of injections, observed backpressures, and column temperature. The system
is controlled by either Empower or MassLynx chromatography/ mass spectrometry
software. Waters - www.waters.com
Normalized
cDNA Library Services
Solid
reagent option for microwave flow reactor
Sample
preparation. 
Protein
purification. 
Discovery
system. 
Antioxidant
assay. 
Platform. 
Evaporator. 
Protein. 
Bioreactor. 
Microplate
cytometer. 
Brochure.